Scientists Discover Genetic Mechanism Essential To Female Development

Friday, Nov 6, 2015

Scientists at The Hebrew University of Jerusalem’s Faculty of Medicine have announced a discovery that is expected to allow doctors to diagnose a disease causing infertility and lack of puberty in women, with implications for the development of future treatment options.

The research paper was chosen as the cover story for the November issue of the prestigious scientific magazine Journal of Clinical Investigation, and was accompanied by a special commentary written by two world-renowned experts in the fields of Drosophila and human reproductive systems.

The mammalian ovary functions not only as the reproductive organ that contains the germ cells (oocytes) responsible for procreating, but also as a hormonal gonad (reproductive gland) regulating many aspects of female physiology and development. Very little is known about the processes involved in the development of the ovary and the egg. To date, only a few genes have been identified as important in ovarian development, and any findings in this area are of great importance with regard to the fields of infertility and fertility.

Researchers discovered a consanguineous family (descended from a common ancestor) where four female cousins presented with ovarian dysgenesis (a lack of ovarian development), though each possessed two X chromosomes, as normal women do. As a result of their lack of sex hormones, these girls did not undergo the regular process of puberty. The women were all treated with replacement hormones, which allowed them to reach a normal height and to experience a monthly period.

Lead researchers on the project were Dr. Offer Gerlitz (Ph.D.), Dept. of Developmental Biology and Cancer Research, Institute for Medical Research, The Hebrew University Faculty of Medicine; Professor David Zangen (MD), Head of Pediatric Endocrinology at Hadassah Medical Center (Mt. Scopus); and Professor Ephrat Levy-Lahad (MD), Professor of Internal Medicine and Medical Genetics at Hebrew University and Director of the Medical Genetics Institute at Shaare Zedek Hospital.

Homozygosity mapping of the girls’ genomes (searching for regions of genetic commonality among them but not shared by their healthy relatives) and sequencing of the entirety of their coding genomes was performed. Using these methods, researchers identified a mutation in the gene Nucleoporin 107 (Nup107), differentiating between the affected patients and their healthy relatives. This gene codes for a protein which is an important component of the channel that connects between the cell’s nucleus (where the DNA is situated) and its cytoplasm (the remainder of the cell’s parts).

Dr. Offer Gerlitz at The Hebrew University explains: “The characterization of the Nup107 gene and discovering its function in ovarian development will provide a greater understanding of how signaling between the cytoplasm and the nucleus is uniquely involved in ovarian development. On a medical level, it will allow the diagnosis of both patients and carriers of the disease, as well as prenatal diagnosis and prevention of this difficult morbidity of lack of puberty and infertility. Potentially, it could also lead to a method of treatment for problems of infertility and premature ovarian failure.”

This Drosophila model system of ovarian dysgenesis was developed using a combination of very advanced genetic and molecular techniques common in Drosophila research. In the opinion of Dr. Gerlitz, this system will allow for the identification of signaling pathways and new genes crucial in ovarian and oocyte development. Therefore, this research will have widespread implications, in the field of infertility as well as in many others, in medicine and in cell biology.

The research is a product of collaboration between the labs of Dr. Gerlitz, a researcher at The Hebrew University, and Professors Zangen and Levy-Lahad from Hadassah and Shaare Zedek, respectively. The labs of Professors Zangen and Levy-Lahad identified the consanguineous family with ovarian dysgenesis and discovered the candidate mutation and gene, while the lab of Dr. Gerlitz generated the disease’s model in Drosophila. This model, on the one hand, proved the involvement of the mutation and the Nup107 gene in the ovarian dysgenesis. On the other hand, it will allow future research into the identification of new signaling pathways and genes involved in ovarian development.